The 'Statistic' option enables you to calculate the frequency of a population (Freq.), the Coefficient of Variation (CV) and the Median Fluorescence Intensity (MFI). How the main window is designed not only allows you to explore the main functionality of FlowJo, but enables you to understand how to correctly determine the concentration of a solution by titration.įlowJo utilizes an array of advanced gating tools that facilitate the process generating subsets until a collection consists of only the cells for which a 3D diagram or analyzed statistics are required. For each sample, you can perform various actions like adding keywords or statistics, display data sets in a spatial spinnable visualization window and concatenate files together. By right-clicking a sample, you can modify group settings, including name, color, font style, role, sample inclusion criterion and assignments. The Group section allows you to organize samples in the order in which you want to analyze them. Moreover, you can drag and drop samples or bands on the workspace. The application provides you with the means to customize each band with the preferred tasks that you commonly use. The interface of FlowJo is user-friendly and consists of the main workspace and several ribbons that contain tabbed bands which allow you to scroll through various bands to access different tasks. Performing a flow cytometry analysis is crucial to diagnose health disorders, especially blood cancers, but it can be helpful to other specialized applications for research and clinical practice purposes.įlowJo is a powerful application that provides you with an integrated environment for displaying and analyzing flow cytometric data.

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